Anti-Thrombin 3 (ATIII) Antigen
Order Name
THROMB3 AG
Test Number: 1500600
Revision Date 05/14/2025
Test Number: 1500600
Revision Date 05/14/2025
| Test Name | Methodology | LOINC Code |
|---|---|---|
|
Anti-Thrombin 3 (ATIII) Antigen
|
Automated (LIATEST) enzyme immunoassay (EIA) | 3175-7 |
| SPECIMEN REQUIREMENTS | ||||
|---|---|---|---|---|
| Specimen | Specimen Volume (min) | Specimen Type | Specimen Container | Transport Environment |
| Preferred | 2 mL (1 mL) | Double Spun Plasma | Sodium Citrate 3.2% (Blue Top) | Frozen |
| Instructions | Volume: 2mL (1mL minimum Note: This volume does not allow for repeat testing.) Collection: Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples unless the sample is collected using a winged (butterfly) collection system. With a winged blood collection set a discard tube should be drawn first to account for the dead space of the tubing and prevent under-filling of the evacuated tube.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternative anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume. Do not pool aliquots together! Stability Requirements: Room temperature n/a, Refrigerated n/a, Frozen 28 days (Freeze/thaw cycles Stable x3) Patient Preparation: Do not draw from an arm with a heparin lock or heparinized catheter. Cause for Rejection: Severe hemolysis; improper labeling; clotted specimen; specimen diluted with IV fluids; samples thawed in transit; improper sample type; sample out of stability |
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| GENERAL INFORMATION | |
|---|---|
| Testing Schedule | Tue |
| Expected TAT | 3-7 Days |
| CPT Code(s) | 85301 |
| Internal Comments | Patient should abstain from anabolic steroids, gemfibrozil, warfarin (coumadin), heparin therapy, asparaginase, estrogens, gestodene, and oral contraceptives optimally for 3 days prior to specimen collection. Overnight fasting is preferred. Each 2.7mL Sodium Citrate 3.2% (Blue Top) tube must be filled to the proper level, no hemolysis. Improperly filled tubes can give erroneous results. The specimen must be double spun then 1.5 mL plasma aliquot from each tube into individual plastic aliquot tubes and freeze within 1 hour of collection. Do not pool aliquots together! Do not thaw. |
| Lab Section | Reference Lab |