Test Number: 6907557
Revision Date 05/19/2020
|Test Name||Methodology||LOINC Code|
SARS-CoV-2 by PCR
|Specimen||Specimen Volume (min)||Specimen Type||Specimen Container||Transport Environment|
|Preferred||1-3mL (0.8mL)||NP Swab||VTM, UTM, ESwab, M6 or Sterile Saline (RML prepared)||Refrigerated|
|Alternate 1||1-3mL (0.8mL)||OP Swab||APTIMA Multitest Swab kit||Room Temp. / Refrigerated|
|Instructions|| Specimen: Nasopharyngeal (NP) swab in VTM, UTM, or M6, ESwab, or sterile saline (RML prepared kits) is the preferred specimen due to superior sensitivity. Oropharyngeal (OP) collections are also acceptable, but may have decreased sensitivity due to decreased presence of virus particles in the OP vs NP. OP or NP washes/aspirates and bronchial washings or bronchoalveolar lavage (BAL) specimens in a minimal volume of sterile saline (2 mL or less preferred) will be accepted, but results must be interpreted with caution due to significantly reduced sensitivity. Nasal swabs have the least sensitivity but may be submitted, so both nares should be generously sampled with a single swab to increase likelihood of adequate virus collection.
Storage / Transport: Refrigerated specimens preferred (if received for testing within 72 hours of collection), if there will be a delay in testing, freeze specimen at -20'C.
Any swab of appropriate size and configuration with a synthetic or rayon tip and plastic shaft can be used.
Do NOT submit swabs with calcium alginate tips or swabs with wood shafts.
Causes for rejection: Swabs with calcium alginate tips; swabs with wooden shafts; refrigerated samples greater than 72 hours old; room temperature swabs greater than 24 hours old; improperly labeled; grossly contaminated; broken or leaking tube; collection with substances inhibitory to PCR including heparin, hemoglobin, ethanol, EDTA concentrations >0.01M; unacceptable transport media.
Swabs must be placed in liquid media, broken or cut at the top of the tube, and tightly capped. Specimen tubes should be labeled with two unique patient identifiers. Place the tube containing the swab in a biohazard bag and submit with paper requisition or place electronic order. Please keep specimen refrigerated until pick-up. Couriers will dispatch to Regional Medical Lab. Call RML at 918-744-2232 to request specimen pickup if not routinely scheduled at your location.
Specimen: Oropharyngeal (OP) swab or anterior nares collections is suggested for pre-surgical testing or patients in a non-hospital setting. All other previously approved media and swabs are still valid for testing in addition to this new media. Click Here to View Collection Kit Information
After OP and Anterior Nares Collection: Immediately place the swab into the transport tube so that the score line is at the top of the tube. Carefully break the swab shaft at the score line against the side of the tube. Throw away the top of the swab, and close the tube.
Storage / Transport: OP Swab kits can be kept at room temperature or refrigerated. Do not freeze.
Stability: 2'C to 30'C up to 6 days.
|Expected TAT||36-72 hours from receipt of specimen in lab.|
|Clinical Use||Detects the 2019 novel coronavirus (SARS-CoV-2).|
|Notes|| Testing for the novel Coronavirus (SARS-CoV-2/COVID-19) is now live at RML core lab.
Client Alert - May14th 2020
Please be aware SARS CoV2 PCR volumes are increasing daily and in the interest of ensuring that we meet the needs of all of our clients, please allow 36-72 hours from receipt of specimens in the lab for results to be available. This applies to all asymptomatic elective surgery specimens. It is also important for elective surgeries that specimen collection be performed 72 hours in advance of the surgery. Please check the Coronavirus testing page often for updates as RML is working hard to add additional SARS CoV2 PCR capacity. Thank you for your cooperation.
|CPT Code(s)|| CPT: 87635
|Lab Section||Molecular Diagnostic|